CBSE Class 10 Science 2019 · Paper 31/4/1 · Question 26(a)
Write four sequential steps of the procedure of the experiment “Preparing a temporary mount of a leaf peel to show stomata.”
Answer
Take a thin peel, stain it with safranin, add glycerine, then lower a cover slip and observe under the microscope.
(1) Take out a thin peel from the lower surface of a fresh leaf and place it in a drop of water on a clean slide.
(2) Stain the peel with a drop of safranin solution; after a minute or two remove the extra stain.
(3) Put a drop of glycerine on the peel.
(4) Place a cover slip gently over it with the help of a needle, avoiding air bubbles, remove the extra glycerine with blotting paper, and observe the slide under the microscope.
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