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CBSE Class 10 Science 2020 · Paper 31/5/2 · Question 28

Life Processes 5 marks Medium
Design an activity to show that chlorophyll is essential for photosynthesis.

Answer

A destarched variegated leaf kept in sunlight and tested with iodine turns blue-black only where it was green, so chlorophyll is essential.

Take a potted plant with variegated leaves; keep it in a dark room for three days to use up its starch, then in sunlight for about six hours. Pluck a leaf and trace its green areas on paper. Dip it in boiling water for a few minutes, then heat it in alcohol placed in a water bath until the alcohol boils (the chlorophyll dissolves). Dip it in dilute iodine solution for a few minutes and rinse it. Observation: only the parts that were green turn blue-black. Inference: starch, made by photosynthesis, forms only where chlorophyll is present, so chlorophyll is essential for photosynthesis.

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